Novel Glyconanoarrays with Specific-Lectin Affinity

Rachid Baati
University of Strasbourg, Faculty of Pharmacy CNRS/UMR 7199, 74 route du Rhin 674001 Illkirch, France.

Abstract:

Introduction:
All the cells are coated with complex carbohydrates called glycans, which form a layer know as the glycocalix. This dense structural informations is decoded by carbohydrate-binding proteins, which are involved in important physiological and pathophysiological events. Despite the increasing awareness of the biological significance of carbohydrates, the study of carbohydrates-proteins interactions still encounters much difficulty. Glycoarrays emerged as a powerful tools for studying such specific interactions. In the frame of our project we describe here an original bottom-up approach relying on the non covalent functionalization of carbon nanotubes for the generation of surface glyco-engineered nanomaterials that interact specifically with lectines.




Oligosaccharides have been immobilized by various methods, which can be classified into three categories: i) physisorption, ii) chemical ligation or chemical graphting and iii) specific biological-based interactions such as biotin/streptavidine assembly.

Our developed bottom-up approach relies first, on the synthesis of new polymerizable glycomimetic that self-assemble via hydrophobic interaction on carbon nanotube surface (Figure 1).^[1,2] The selective photopolymerization of diynes groups generates glyconanorings and stable multivalent platforms densely covered by sugar epitopes, that exhibit specific binding with PNA lectines.^[2]



We have demonstrated that the newly synthesized bionanomaterials can be engaged in specific lectine binding affinity events. The details of our studies will be reported in the poster. ^[3,4]

References: [1] C. Richard, F. Balavoine, P. Schultz, T. W. Ebbesen, C. Mioskowski Science 2003, 300, 775;
[2] N. Khiar*, M. Pernia, R. Baati*, C. Ruhlmann, C. Mioskowski, P. Schultz, I. Fernandez. Chem. Com. 2009, 4121 ;
[3] M. Assali, M. Pernia, I. Fernandez, R. Baati,  C. Mioskowski, N. Khiar Soft Matter, 2009, 5, 948; M. Assali, M. P. Leal, I. Fernadez, P. Romero-Gomez, R. Baati, N. Khiar Nano. Res. 2010, 3, 764-778.